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發(fā)布時(shí)間:2023-07-12 發(fā)布人:天合·種電 發(fā)布來源:http://www.51dwgw.cn/
1.一種利用復(fù)合微生物菌劑凈化海水養(yǎng)殖廢水的方法,其特征在于包 括如下步驟:
1. A method for purifying seawater aquaculture wastewater using a composite microbial agent, characterized in that it comprises the following steps:
1)篩選復(fù)合微生物菌劑:由光合細(xì)菌、硝化細(xì)菌、反硝化細(xì)菌、嗜酸 乳酸菌、產(chǎn)朊假絲酵母以及芽孢桿菌組成復(fù)合微生物菌劑;
1) Screening of composite microbial agents: composite microbial agents are composed of photosynthetic bacteria, Nitrifying bacteria, denitrifying bacteria, acidophilic lactic acid bacteria, Candida utilis and bacillus;
2)復(fù)合微生物菌劑采用培養(yǎng)基活化:在培養(yǎng)基中按1%接種量(w/v) 直接投加上述復(fù)合微生物菌劑,在37℃搖床150rpm下活化菌種24h, 使微生物總含量為7×108~9×108CFU/mL;
2) The composite microbial inoculum is activated using a culture medium: the above composite microbial inoculum is directly added to the culture medium at a rate of 1% inoculation (w/v), and activated at a shaking table of 150 rpm at 37 ℃ for 24 hours, resulting in a total microbial content of 7 × 108~9 × 108CFU/mL;
3)活化后的復(fù)合菌劑投菌量按所處理養(yǎng)殖廢水0.6‰~1.2‰比例進(jìn)行 投加。
3) The activated composite bacterial agent is added in a ratio of 0.6 ‰ to 1.2 ‰ for the treated aquaculture wastewater.
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2.如權(quán)利要求1所述的一種利用復(fù)合微生物菌劑凈化海水養(yǎng)殖廢水的 方法,其特征在于:所述的步驟1)中復(fù)合微生物菌劑中的五種菌種培養(yǎng) 后,根據(jù)被處理的海水養(yǎng)殖廢水的成份進(jìn)行調(diào)節(jié)、配制成復(fù)合微生物菌劑, 使菌劑中微生物總菌數(shù)≥1.0×1010CFU/g,且其中光合細(xì)菌≥5.0×108CFU/g,硝化細(xì)菌≥2.0×108CFU/g,反硝化細(xì)菌≥3.0×108CFU/g,嗜酸 乳酸菌≥1.0×109CFU/g,產(chǎn)朊假絲酵母≥5.0×108CFU/g,芽孢桿菌≥ 7.5×109CFU/g。
2. A method for purifying seawater aquaculture wastewater using a composite microbial agent as claimed in claim 1, characterized in that: in step 1), five strains of the composite microbial agent are cultured and adjusted according to the composition of the treated seawater aquaculture wastewater to form a composite microbial agent, so that the total number of microorganisms in the agent is ≥ 1.0 × 1010CFU/g, with photosynthetic bacteria ≥ 5.0 × 108CFU/g, Nitrifying bacteria ≥ 2.0 × 108CFU/g, denitrifying bacteria ≥ 3.0 × 108CFU/g, acidophilic lactic acid bacteria ≥ 1.0 × 109CFU/g, Candida albicans ≥ 5.0 × 108CFU/g, Bacillus subtilis ≥ 7.5 × 109CFU/g.
3.如權(quán)利要求2所述的一種利用復(fù)合微生物菌劑凈化海水養(yǎng)殖廢水的 方法,其特征在于:所述的五種菌種分別采用微生物菌種資源庫保藏 號為CCTCC M 205147的沼澤紅假單胞、ACCC 10691的節(jié)桿菌、ACCC 03247 的反硝化產(chǎn)堿菌、ACCC 10637的嗜酸乳酸菌、CGMCC 2.1016的產(chǎn)朊假絲酵 母、CGMCC 1.1630的枯草芽孢桿菌,進(jìn)行耐鹽、耐溫馴化,作為生產(chǎn)菌, 組成復(fù)合微生物菌劑。
3. A method for purifying seawater aquaculture wastewater using a composite microbial agent as claimed in claim 2, characterized in that the five bacterial strains are respectively composed of Rhodopseudomonas mirabilis with the preservation number CCTCC M 205147, Arthrobacter spp. with ACCC 10691, denitrifying alkali producing bacteria with ACCC 03247, acidophilic lactic acid bacteria with ACCC 10637, Candida albicans with CGMCC 2.1016, and Bacillus subtilis with CGMCC 1.1630, Conduct salt and temperature tolerance domestication as a production bacterium to form a composite microbial agent.
4.如權(quán)利要求1所述的一種利用復(fù)合微生物菌劑凈化海水養(yǎng)殖廢水的 方法,其特征在于所述的步驟2)中培養(yǎng)基組分為:蛋白胨3g/L、酵母粉 3g/L、葡萄糖5g/L、氯化鈉10g/L、磷酸二氫鉀0.5g/L、七水硫酸鎂 0.2g/L、硫酸銨1g/L、海水1L,調(diào)pH 7.0。
4. The method for purifying mariculture wastewater by using a composite microbial agent as claimed in claim 1, which is characterized in that in step 2), the composition of the culture medium is: peptone 3g/L, yeast powder 3g/L, glucose 5g/L, sodium chloride 10g/L, Monopotassium phosphate 0.5g/L, magnesium sulfate heptahydrate 0.2g/L, ammonium sulfate 1g/L, seawater 1L, and the pH is adjusted to 7.0.
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